Immunoassays play a significant role in the medicine and research world. Through immunoassay tests, clinicians have sped up service provisions to patients, shortening hospital stays and decreasing the severity of illness. This is due to quick identification and assessment of disease, leading to taking necessary measures. There are different types of immunoassays tests and tools used for analysis. Immunoassay kits are well-designed tools aimed at accelerating the research process. However, all the immunoassay tools differ in function, speed, and sensitivity. Understanding the differences will help you determine the best assay kits that you can choose to use.
In this article, we will focus on ELISA Kits, surveying the various advantages associated with these kits.
What are ELISA Kits?
ELISA kits are tools created to detect various proteins and molecules such as peptides, markers for infectious diseases, drugs, and hormones. Elisa Kits are well designed, making them quick, convenient, and accurate for the research, detection, and quantification of targeted molecules or samples. ELISA kits differ from other immunoassay kits since they provide quantitative and more specific results.
ELISA kits are majorly used for enzyme-linked immunosorbent assay tests, which are carried out to detect and measure the presence of antibodies, peptides, hormones, and protein in the blood. Antibodies are blood proteins synthesized as a response to specific antigens. In case of infection, the ELISA test is used to investigate and analyze the presence of antibodies in the body. ELISA kit is also an effective diagnostic tool in plant research. They can also be used in quality control, especially in food industries.
Using ELISA kits, different ELISA tests can be carried out, including Indirect Elisa, Sandwich Elisa, and Competitive Elisa. All these tests differ in where the Antigen and antibodies are coated or placed when conducting research.
Antigen-antibody concentration can be measured effectively using ELISA. ELISA works under a simple principle: the target antigen is bound by particular antibodies, which also count the number of antigens bound. The plate must be coated with antibodies with high affinity to maximize the assay's sensitivity and accuracy.